locus

BSU08690

BSU_08690

outlinks

bsu

BSU08690

BSU_08690

The protein

[SW|Domains]

has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]

[SW|ABC transmembrane type-1 domain] (aa 19-307) (according to UniProt)

[SW|ABC transporter domain] (aa 341-576) (according to UniProt)

Biological materials

Mutant

MGNA-A244 (ygaD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/244 NBRP B. subtilis, Japan]

BKE08690 ([[gene|ygaD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGGTTGATTCCCCCTCTC, downstream forward: _UP4_TAAAACATCAATCTTATATC

BKK08690 ([[gene|ygaD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGGTTGATTCCCCCTCTC, downstream forward: _UP4_TAAAACATCAATCTTATATC

MGNA-A244 (ygaD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/244 NBRP B. subtilis, Japan]

BKE08690 ([[gene|ygaD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08690 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CACGGTTGATTCCCCCTCTC, downstream forward: _UP4_TAAAACATCAATCTTATATC

BKK08690 ([[gene|ygaD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGGTTGATTCCCCCTCTC, downstream forward: _UP4_TAAAACATCAATCTTATATC

Biological materials

two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab